A two-color acid-free cartilage and bone stain for zebrafish larvae |
| |
Authors: | Mb Walker Cb Kimmel |
| |
Institution: |
a Stowers Institute for Medical Research, Kansas City, Missouri
b University of Oregon, Eugene, Oregon |
| |
Abstract: | Traditionally, cartilage is stained by alcian blue using acidic conditions to differentiate tissue staining. The acidic conditions are problematic when one wishes to stain the same specimen for mineralized bone with alizarin red, because acid demineralizes bone, which negatively affects bone staining. We have developed an acid-free method to stain cartilage and bone simultaneously in zebrafish larvae. This method has the additional advantage that PCR genotyping of stained specimens is possible. |
| |
Keywords: | alcian blue alizarin red PCR genotyping |
本文献已被 InformaWorld 等数据库收录! |
|