Transgenic rice plants harboring the grain hardness-locus region of Aegilops tauschii |
| |
Authors: | Go Suzuki Hideo Wada Hiromi Goto Akiko Nakano Haruna Oba Takuya Deno Sadequr Rahman Yasuhiko Mukai |
| |
Institution: | (1) Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara Osaka, 582-8582, Japan;(2) Division of Practical School Education, Osaka Kyoiku University, 4-88 Minami-Kawahoricho, Tennoji Osaka, 543-0054, Japan;(3) School of Science, Monash University, Sunway Campus, Jalan Lagoon Selatan, Bandar Sunway, 46150 Petaling Jaya, Malaysia |
| |
Abstract: | Grain hardness of wheat is determined by the hardness (Ha)-locus region, which contains three friabilin-related genes: puroindoline-a (Pina), puroindoline-b (Pinb) and GSP-1. In our previous study, we produced the transgenic rice plants harboring the large genomic fragment of the Ha-locus region of Aegilops tauschii containing Pina and GSP-1 genes by Agrobacterium-mediated transformation. To examine the effects of the transgenes in the rice endosperms, we firstly confirmed the homozygosity
of the T-DNAs in four independent T2 lines by using fluorescence in situ hybridization (FISH) and DNA gel blot analyses. The transgenes, Pina and GSP-1, were stably expressed in endosperms of the T3 and T4 seeds at RNA and protein levels, indicating that the promoters and other regulatory elements on the wheat Ha-locus region function in rice, and that multigene transformation using a large genomic fragment is a useful strategy. The
functional contribution of the transgene-derived friabilins to the rice endosperm structure was considered as an increase
of spaces between compound starch granules, resulting in a high proportion of white turbidity seeds. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|