A rapid and efficient DNA minipreparation suitable for screening transgenic plants |
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| Authors: | Rong-Cheng Lin Zai-Song Ding Liang-Bi Li Ting-Yun Kuang |
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| Institution: | (1) Photosynthesis Research Center, Institute of Botany, the Chinese Academy of Sciences, 100093 Beijing, China |
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| Abstract: | We present a modified method for DNA minipreparation suitable for large-scale screening of transgenic plants. The method is
rapid and efficient—one person can prepare DNA from approximately 50 samples per day. The average yield was about 40 μg DNA
per 100 mg of fresh tissue, and the A260/A280 was 1.89–2.03. The total DNA extracted by this method could be used for PCR, restriction enzyme digestion, and Southern blotting. |
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| Keywords: | DNA extraction SDS transgenic plants |
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