The alanine racemase of Mycobacterium smegmatis is essential for growth in the absence of D-alanine |
| |
Authors: | Milligan Daniel L Tran Sieu L Strych Ulrich Cook Gregory M Krause Kurt L |
| |
Institution: | Department of Biochemistry, Otago School of Medical Sciences, University of Otago, P.O. Box 56, Dunedin, New Zealand. |
| |
Abstract: | Alanine racemase, encoded by the gene alr, is an important enzyme in the synthesis of d-alanine for peptidoglycan biosynthesis. Strains of Mycobacterium smegmatis with a deletion mutation of the alr gene were found to require d-alanine for growth in both rich and minimal media. This indicates that alanine racemase is the only source of d-alanine for cell wall biosynthesis in M. smegmatis and confirms alanine racemase as a viable target gene for antimycobacterial drug development. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|