Comparison of a competitive enzyme immunoassay kit and the infant mouse assay for detecting Escherichia coli heat-stable enterotoxin |
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Authors: | S. Stavric B. Buchanan J. Speirs |
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Affiliation: | Bureau of Microbial Hazards, Health & Welfare Canada, Ottawa, Ontario. |
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Abstract: | A commercial competitive enzyme immunoassay kit, Escherichia coli ST EIA, was compared with the conventional infant mouse assay for sensitivity and specificity in detecting E. coli heat-stable enterotoxin. Thirty-one of 46 strains of E. coli tested were positive by both assays, while 15 strains were negative. The sensitivity of the ST EIA kit was up to 64-fold lower than the infant mouse assay. |
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