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A simple lipase assay using trichloroacetic acid |
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| Authors: | M C Schotz A S Garfinkel |
| Abstract: | An extremely rapid and sensitive assay for lipoprotein lipase activity, suitable for routine determinations, is described. The substrate for the assay is emulsified 2-(3)H]glyceryl trioleate, activated by serum. The method is based on trichloroacetic acid precipitation of unreacted substrate and measurement of (3)H-labeled glycerol. |
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