Effect of Freezing and Type of Mucosa on <Emphasis Type="Italic">Ex Vivo</Emphasis> Drug Permeability Parameters |
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Authors: | Thiago Caon Cláudia Maria Oliveira Simões |
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Institution: | 1.Laboratório de Virologia Aplicada, Departamento de Ciências Farmacêuticas,Universidade Federal de Santa Catarina, Campus Universitário,Florianópolis,Brazil |
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Abstract: | The porcine esophageal mucosa has been proposed as a substitute for the buccal mucosa barrier on ex vivo permeability studies mainly due to its large surface area as well as its easier preparation. Therefore, this study compared
the ex vivo permeability parameters of two drugs (carmabazepine (CBZ) and triamcinolone acetonide (TAC)) with different permeabilities
and physicochemical properties through buccal and esophageal mucosae using a Franz diffusion cell system and HPLC as detection
method. The freezing effects on drug permeability parameters were also evaluated by comparing them when fresh and frozen tissues
were used. The barrier properties were not affected by the freezing process since the obtained parameters for both drugs were
similar in frozen and fresh tissues (buccal and esophageal mucosae). However, an increase of CBZ retention was shown in frozen
tissues. Fresh and frozen esophageal mucosae provided higher permeation of TAC than on buccal mucosae while the obtained permeability
parameters for CBZ were similar on both mucosae. According to our results, porcine esophageal mucosa could be used as a substitute
for buccal mucosa on ex vivo studies involving CBZ but not TAC. Frozen tissues could be used as substitute for fresh tissues in both cases. However, any
substitution should be done with care and only if previous tests were performed, because the results could differ depending
on the tested drug. |
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