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The potential of glycerol in freezing preservation of turbine oil-degrading bacterial consortium and the ability of the revised consortium to degrade petroleum wastes |
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| Institution: | 1. Key Laboratory of Experimental Marine Biology, National and Local Joint Engineering Laboratory of Ecological Mariculture, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, Shandong 266071, China;2. Nantong Branch, Institute of Oceanology, Chinese Academy of Sciences, Nantong, Jiangsu 226004, China;3. Qingdao National Laboratory for Marine Science and Technology, Qingdao, Shandong 266200, China |
| Abstract: | The turbine oil (TuO)-degrading bacterial consortium Tank-2 (original Tank-2) was preserved as a glycerol stock at ?80 °C from 2009 to 2012. Storage methods have been unavailable so far for any TuO-degrading bacterial consortia or isolates. To evaluate the usefulness of glycerol stock, the original Tank-2 consortium frozen in glycerol at ?80 °C was thawed and then revived by repeated culture in mineral salts medium (MSM) containing 0.5% (w/w) TuO (revived Tank-2). The revived Tank-2 consortium exhibited a high activity to degrade TuO, which was equivalent to that of original Tank-2. It also degraded car engine oil, used car engine oil, Arabian light and Vityaz crude oils and TuO in wastewater. These results indicated that a glycerol stock at ?80 °C was useful for storing Tank-2. PCR-denaturing gradient gel electrophoresis (DGGE) that targeted the V3 regions of 16S rRNA gene sequences showed that the DGGE band profiles of principal bacteria were significantly different between the original and revived Tank-2 consortia and between the revived Tank-2 culture grown in MSM containing TuO and that grown in MSM containing other types of petroleum products. This suggested that bacterial strains inherently residing in Tank-2 could adjust their compositions based on the storage and culture conditions. |
| Keywords: | Bacterial consortia Biodegradation Crude oil Culture revival Glycerol stock Turbine oil Wastewater DGGE"} {"#name":"keyword" "$":{"id":"kwrd0050"} "$$":[{"#name":"text" "_":"denaturing gradient gel electrophoresis EO"} {"#name":"keyword" "$":{"id":"kwrd0060"} "$$":[{"#name":"text" "_":"engine oil ICP-AES"} {"#name":"keyword" "$":{"id":"kwrd0070"} "$$":[{"#name":"text" "_":"inductively coupled plasma-atomic emission spectrometry MSM"} {"#name":"keyword" "$":{"id":"kwrd0080"} "$$":[{"#name":"text" "_":"minimal salts medium TLC-FID"} {"#name":"keyword" "$":{"id":"kwrd0090"} "$$":[{"#name":"text" "_":"thin-layer chromatography-flame-ionization detection TPH"} {"#name":"keyword" "$":{"id":"kwrd0100"} "$$":[{"#name":"text" "_":"total petroleum hydrocarbon TuO"} {"#name":"keyword" "$":{"id":"kwrd0110"} "$$":[{"#name":"text" "_":"turbine oil |
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