Sphingobium sp. FB3 degrades a mixture of polycyclic aromatic hydrocarbons |
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| Institution: | 1. College of Resources and Environmental Sciences, China Agricultural University, 2 Yuanmingyuan west road, Beijing 100193, China;2. Department of Molecular Biosciences and Bioengineering, University of Hawaii, 1955 East-West Road, Honolulu, HI 96822, USA;3. Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, 6 Tongwei Road, Nanjing 210095, China;1. Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;1. State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No. 1 West Beichen Road, Chaoyang District, Beijing 100101, China;2. School of Environmental Science, Tsinghua University, Beijing 100084, China;3. Environmental Protection Research Institute of Light Industry, Beijing 100089, China;1. Center of Excellence in Environmental Studies, King Abdulaziz University, Jeddah 21589, Saudi Arabia;2. Laboratorie de Biotechnologie de l’Environnement, Institut National de la Recherche Agronomique, Avenue des Etangs, 11100, Narbonne, France;1. Environmental Engineering Department of Nanjing Institute of Technology, 211167, Nanjing, PR China;2. Environmental Engineering Department of Hohai University, 210098, Nanjing, PR China;1. Institute of Soil and Water Resources and Environmental Science, College of Environmental and Resource Sciences, Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment, Zhejiang University, Hangzhou, 310058, China;2. Department of Environmental Sciences, University of California, Riverside, CA 92521, USA |
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| Abstract: | A soil sample collected underneath a sewage pipe of the west side of Yangpu refining factory in Haikou city, Hainan Province, China was inoculated in minimum medium supplemented with fluoranthene. After 8 enrichment cycles, a bacterial consortium (Y12) was obtained through water-silicone oil dual system in the laboratory. The consortium Y12 could degrade a mixture of polycyclic aromatic hydrocarbons (PAHs) including phenanthrene, anthracene, fluoranthene, pyrene and benzoa]pyrene. The consortium Y12 was repeatedly cultured for more than 40 circles, from which a bacterial strain FB3 was isolated. This strain was identified as a Sphingobium sp. through the 16S rDNA sequence alignment. Strain FB3 could degrade 99 ± 0.4%, 67 ± 2%, 97 ± 3%, 72 ± 8%, and 6 ± 2% (uncorrected degradation percentages) of phenanthrene, anthracene, fluoranthene and pyrene each at level of 100 mg L?1 and benzoa]pyrene at 10 mg L?1, respectively, in 10 days, which the five PAHs were the sole carbon source as a mixture in minimum medium. The degradation percentages of phenanthrene, anthracene, fluoranthene, pyrene (each at level of 100 mg L?1) and benzoa]pyrene (10 mg L?1) by consortium Y12 were 99 ± 0.1%, 65 ± 3%, 99 ± 0.3%, 79 ± 1% and 7 ± 6%, respectively, in 10 days. Strain FB3 could degrade those PAHs under a range of pH 5–9, being optimum at pH 7. |
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| Keywords: | Mixed PAHs Consortium Degradation |
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