Salvianolic acid B promotes osteogenesis of human mesenchymal stem cells through activating ERK signaling pathway |
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Affiliation: | 1. Institute of Orthopedic Surgery, Xijing Hospital, Fourth Military Medical University, Xi''an 710032, People''s Republic of China;2. Air Force General Hospital, People''s Liberation Army, Beijing 100142, People''s Republic of China |
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Abstract: | Salvianolic acid B, a major bioactive component of Chinese medicine herb, Salvia miltiorrhiza, is widely used for treatment of cardiovascular diseases. Our recent studies have shown that Salvianolic acid B can prevent development of osteoporosis. However, the underlying mechanisms are still not clarified clearly. In the present study, we aim to investigate the effects of Salvianolic acid B on viability and osteogenic differentiation of human mesenchymal stem cells (hMSCs). The results showed Salvianolic acid B (Sal B) had no obvious toxic effects on hMSCs, whereas Sal B supplementation (5 μM) increased the alkaline phosphatase activity, osteopontin, Runx2 and osterix expression in hMSCs. Under osteogenic induction condition, Sal B (5 μM) significantly promoted mineralization; and when the extracellular-signal-regulated kinases signaling (ERK) pathway was blocked, the anabolic effects of Sal B were diminished, indicating that Sal B promoted osteogenesis of hMSCs through activating ERK signaling pathway. The current study confirms that Sal B promotes osteogenesis of hMSCs with no cytotoxicity, and it may be used as a potential therapeutic agent for the management of osteoporosis. |
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Keywords: | Mesenchymal stem cell Osteogenesis Salvianolic acid B Osteoporosis |
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