Reconstructed mung bean trypsin inhibitor targeting cell surface GRP78 induces apoptosis and inhibits tumor growth in colorectal cancer |
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Affiliation: | 1. Section of Pharmacology, Department of Pharmacy & Drug Sciences, University of Bari “Aldo Moro”, Via E. Orabona, 4, 70125 Bari, Italy;2. Department of Molecular Medicine, University of Pavia, Via C. Forlanini, 6, 27100 Pavia, Italy;3. Department of Chemistry and SMART Inter-department Research Center, University of Bari “Aldo Moro”, Via E. Orabona 4, 70125 Bari, Italy;4. National Research Council (CNR)—Institute of Chemistry of Organometallic Compounds (ICCOM) Bari Section, Via E. Orabona 4, 70125 Bari, Italy;5. Department of Chemistry, University of Bari “Aldo Moro”, Via E. Orabona 4, 70125 Bari, Italy;6. Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari “Aldo Moro”, Via E. Orabona, 4, 70125 Bari, Italy |
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Abstract: | Glucose regulated protein 78 (GRP78) has been reported to be present on cell membranes of cancer cells but not the normal cells, serving as a potential anti-cancer target. In the present study, a fusion protein containing the GRP78 binding peptide WIFPWIQL and the active fragment of mung bean trypsin inhibitor was constructed, and its targeted anti-tumor effects were investigated both in vitro and in vivo. The results showed that the fusion protein specifically inhibited growth and induced apoptosis in colorectal cancer cells but not in the normal cells. Mechanistically, these anti-tumor effects were attributed to induction of G1 phase arrest and activation of multiple apoptotic pathways. Importantly, the fusion protein could also suppress the growth of xenografted human colorectal carcinoma in vivo. Our study reveals that this fusion protein may be developed as a therapeutic agent for treatment of colon cancer, and holds important implications for developing other anti-cancer peptide drugs. |
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Keywords: | Trypsin inhibitor GRP78 Colon cancer Targeted anti-tumor effect |
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