Metabolic engineering of Escherichia coli for efficient free fatty acid production from glycerol |
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| Institution: | 1. Department of Bioengineering, Rice University, Houston, TX, USA;2. Technology Holding, Salt Lake City, UT, USA;3. Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX, USA;1. Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, 121-742, Republic of Korea;2. Korea C1 Gas Refinery R&D Center, Sogang University, Seoul, 121-742, Republic of Korea;3. Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Yuseong-gu, Daejeon, 305-701, Republic of Korea;4. Department of Food Science and Engineering, Ewha Womans University, Seoul, 120-750, Republic of Korea;1. Bioprocessing and Renewable Energy Laboratory, Department of Grain Science and Industry, Kansas State University, Manhattan, KS, USA;2. Department of Chemical Engineering, The University of Utah, Salt Lake City, UT, USA;3. Technology Holdings LLC, Salt Lake City, UT, USA;4. Department of Chemical Engineering, Kansas State University, Manhattan, KS, USA;1. Institut Pasteur, Unité de Chimie et Biocatalyse, 28 rue du Dr Roux, 75724, Paris cedex 15, France;2. CNRS, UMR3523, Paris, France;3. INSERM, U759, Institut Curie, 91405, Orsay, France;4. CNRS, UMR5048, Université Montpellier, Centre de Biochimie Structurale, 29, route de Navacelles, 34090, Montpellier, France;5. INSERM, U1054, Montpellier, France;6. Institut Pasteur, Unité des Interactions Bactéries-Cellules, 25 rue du Dr Roux, 75724 Paris cedex 15, France;7. INSERM, U604, Paris, France;8. INRA, USC2020, Paris, France;9. Université Paris Diderot, Sorbonne Paris Cité, Paris, France |
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| Abstract: | Crude glycerol, generated as waste by-product in biodiesel production process, has been considered as an important carbon source for converting to value-added bioproducts recently. Free fatty acids (FFAs) can be used as precursors for the production of biofuels or biochemicals. Microbial biosynthesis of FFAs can be achieved by introducing an acyl–acyl carrier protein thioesterase into Escherichia coli. In this study, the effect of metabolic manipulation of FFAs synthesis cycle, host genetic background and cofactor engineering on FFAs production using glycerol as feed stocks was investigated. The highest concentration of FFAs produced by the engineered stain reached 4.82 g/L with the yield of 29.55% (g FFAs/g glycerol), about 83% of the maximum theoretical pathway value by the type II fatty acid synthesis pathway. In addition, crude glycerol from biodiesel plant was also used as feedstock in this study. The FFA production was 3.53 g/L with a yield of 24.13%. The yield dropped slightly when crude glycerol was used as a carbon source instead of pure glycerol, while it still can reach about 68% of the maximum theoretical pathway yield. |
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| Keywords: | Fatty acid Glycerol Metabolic engineering Redox bioavailability |
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