Utilizing an endogenous pathway for 1-butanol production in Saccharomyces cerevisiae |
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| Institution: | 1. Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA;2. Departments of Chemistry, Biochemistry, and Bioengineering, Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA;1. Center for Nanodiagnostics, Department of Physical and Colloid Chemistry, I. M. Gubkin Russian State University of Oil and Gas, 119991 Moscow, Russian Federation;2. Center for Advanced Biomedical Imaging and Photonics, Beth Israel Deaconess Medical Center, Harvard University, Boston, Massachusetts 02215, USA;1. INRA, UMR1083, 2 Place Viala, Montpellier Cedex 1 F-34060, France;2. Universite Montpellier, UMR1083, 2 Place Viala, Montpellier Cedex 1 F-34060, France;3. AgroParisTech, INRA, UMR 782, Thiverval-Grignon F-78850, France;1. Department of Chemical and Biomolecular Engineering, Carl R Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, IL 61801, United States;2. Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue , Urbana, IL 61801, United States;3. Department of Biochemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue , Urbana, IL 61801, United States;4. Department of Bioengineering, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue , Urbana, IL 61801, United States;1. Department of Biotechnology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan 81746-73441, Iran;2. Environmental Research Institute, University of Isfahan, Isfahan 81746-73441, Iran;1. Department of Biomass and Leather Engineering, Sichuan University, Chengdu 610065, PR China;2. The Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu 610065, PR China;1. CAS Key Laboratory of Renewable Energy, Guangzhou, 510640, People''s Republic of China;2. Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou, 510640, People''s Republic of China;3. Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou, 510640, People''s Republic of China;4. University of Chinese Academy of Sciences, Beijing, 100049, People''s Republic of China;5. National Research Base of Intelligent Manufacturing Service, Chongqing Technology and Business University, Chongqing, 400067, People''s Republic of China |
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| Abstract: | Microbial production of higher alcohols from renewable feedstock has attracted intensive attention thanks to its potential as a source for next-generation gasoline substitutes. Here we report the discovery, characterization and engineering of an endogenous 1-butanol pathway in Saccharomyces cerevisiae. Upon introduction of a single gene deletion adh1Δ, S. cerevisiae was able to accumulate more than 120 mg/L 1-butanol from glucose in rich medium. Precursor feeding, 13C-isotope labeling and gene deletion experiments demonstrated that the endogenous 1-butanol production was dependent on catabolism of threonine in a manner similar to fusel alcohol production by the Ehrlich pathway. Specifically, the leucine biosynthesis pathway was engaged in the conversion of key 2-keto acid intermediates. Overexpression of the pathway enzymes and elimination of competing pathways achieved the highest reported 1-butanol titer in S. cerevisiae (242.8 mg/L). |
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| Keywords: | 1-Butanol Metabolic engineering Biofuel |
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