Production of anteiso-branched fatty acids in Escherichia coli; next generation biofuels with improved cold-flow properties |
| |
| Affiliation: | 1. Joint BioEnergy Institute, 5885 Hollis Street, 4th Floor, Emeryville, CA 94608, United States;2. Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, United States;3. QB3 Institute, University of California-Berkeley, 5885 Hollis Street, 4th Floor, Emeryville, CA 94608, United States;4. Synthetic Biology Engineering Research Center, University of California, Berkeley, CA 94720, United States;5. Department of Chemical & Biomolecular Engineering, Department of Bioengineering, University of California, Berkeley, CA 94720, United States;1. Department of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, Saint Louis, MO 63130, USA;2. Division of Biological & Biomedical Sciences, Washington University in St. Louis, Saint Louis, MO 63130, USA;3. Institute of Materials Science & Engineering, Washington University in St. Louis, Saint Louis, MO 63130, USA;1. Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, China;2. Hubei Engineering Laboratory for Synthetic Microbiology, Wuhan Institute of Biotechnology, Wuhan 430075, China;1. Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Environmental Microbial Technology Center of Hubei Province, College of Life Sciences, Hubei University, Wuhan 430062, PR China;2. College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, PR China;3. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China;4. Department of Food Science and Human Nutrition, Iowa State University, Ames, IA 50011, USA;1. DOE Joint BioEnergy Institute, Emeryville, CA 94608, United States;2. Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, United States;3. Biomass Science and Conversion Technology Department, Sandia National Laboratories, Livermore, CA 94551, United States;4. Biological and Materials Science Center, Sandia National Laboratories, Livermore, CA 94551, United States;5. Department of Chemical & Biomolecular Engineering, University of California, Berkeley, CA 94720, United States;6. Department of Bioengineering, University of California, Berkeley, CA 94720, United States;7. The Novo Nordisk Foundation Center for Sustainability, Technical University of Denmark, Denmark;1. Department of Environmental Science and Engineering, School of Petrochemical Engineering & Environment, Zhejiang Ocean University, Zhoushan 316000, China;2. Zhejiang Key Laboratory of Petrochemical Environmental Pollution Control, Zhoushan 316000, China |
| |
| Abstract: | Microbial fermentation is emerging as an increasingly important resource for the production of fatty acids to serve as precursors for renewable diesel as well as detergents, lubricants and other industrial chemicals, as an alternative to traditional sources of reduced carbon such as petroleum. A major disadvantage of fuels derived from biological sources is their undesirable physical properties such as high cloud and pour points, and high viscosity. Here we report the development of an Escherichia coli strain that efficiently produces anteiso-branched fatty acids, which can be converted into downstream products with lower cloud and pour points than the mixtures of compounds produced via the native metabolism of the cell. This work addresses a serious limitation that must be overcome in order to produce renewable biodiesel and oleochemicals that perform as well as their petroleum-based counterparts. |
| |
| Keywords: | Biofuels Synthetic biology Fatty acids |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
