Biodegradation of phenanthrene by Pseudomonas sp. BZ-3, isolated from crude oil contaminated soil |
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| Affiliation: | 1. Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;1. Universidad Tecnológica Nacional (UTN), Facultad Regional de Haédo, París 532, 1706 Haedo, Buenos Aires, Argentina;2. Instituto de Investigaciones para la Industria Química (INIQUI), CONICET-UNSa, Argentina;3. Facultad de Ciencias Naturales, UNSa, Salta, Argentina;4. Centro de Investigaciones y Transferencia de Villa María (CIT Villa María), CONICET-Instituto de Ciencias Básicas y Aplicadas, Universidad Nacional de Villa María, Córdoba, Argentina;5. Planta Piloto de Procesos Industriales Microbiológicos (PROIMI, CONICET), Tucumán, Argentina;6. Instituto de Astronomía y Física del Espacio, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Buenos Aires, Intendente Güiraldes 2160, C1428EGA CABA, Argentina;1. Laboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, University of Sfax, PO Box 1177, 3018, Sfax, Tunisia;2. Center of Sustainable Development, College of Arts and Sciences, Qatar University, 2713, Doha, Qatar;1. Department of Biosciences, UGC Center of Advanced Study, Satellite Campus, Vadtal Road, Sardar Patel University, Bakrol 388 315, Anand, Gujarat, India;2. P D Patel Institute of Applied Sciences, Charotar University of Science and Technology, Charusat Campus, Changa 388 421, Anand, Gujarat, India |
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| Abstract: | A phenanthrene (PHE) degrading bacterium strain BZ-3 was isolated from the crude oil contaminated soil in Binzhou, China. The isolate was identified as Pseudomonas sp. BZ-3 on the basis of 16S rRNA gene sequence. Various experiments were conducted to investigate the effect of pH, salinity and PHE concentration on the degradation efficiency of PHE. The degradation efficiency and degradation metabolites of PHE were detected by using GC–MS and HPLC-MS analyses. The strain BZ-3 could degrade 75% of PHE at an initial concentration of 50 mg/L under 20 g/L salinity in 7 days. PHE degradation kinetics was estimated in a first-order degradation rate model and the rate coefficient was calculated as 0.108 d−1. On the basis of the identified degradation metabolites, the strain BZ-3 could degrade PHE in the salicylate metabolic pathway. In a mixture system consisting of PHE and other PAHs including naphthalene (NA), anthracene (ANTH), and pyrene (PYR), the strain BZ-3 showed an efficiently degradation capability. Further study showed that the strain BZ-3 could also use NA, ANTH, PYR, xylene, 1-hydroxy-2-naphthoic acid, and hexane as the sole carbon and energy source, but did not grow on nitrobenzene-containing medium. |
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| Keywords: | Biodegradation Phenanthrene Halophilic microorganism Metabolic pathway |
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