Diversity of airborne bacteria in samples collected using different devices for aerosol collection |
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Authors: | Camilla Fahlgren Gunnar Bratbak Ruth-Anne Sandaa Runar Thyrhaug Ulla Li Zweifel |
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Institution: | 1.School of Natural Sciences,Linnaeus University,Kalmar,Sweden;2.Department of Biology,University of Bergen,Bergen,Norway;3.Department of Cell and Molecular Biology,University of Gothenburg,G?teborg,Sweden |
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Abstract: | Bacteria are ubiquitous in the atmosphere, where they form a highly diverse community, albeit low in abundance. Several approaches
are available for collecting airborne particles, though few comparative studies have been conducted to date. This study examined
how different sampling strategies affect the apparent composition of the airborne community. Three devices were tested: an
impactor, a liquid impinger, and a Teflon membrane filter. Comparative studies were conducted at one mountainous location
in Norway and one seaside location in Sweden. At both locations, microbial samples were collected in parallel using the sampling
devices. DNA extraction, construction of 16S rRNA gene clone libraries, and subsequent sequencing were used to identify the
bacteria. The comparison between clone libraries retrieved using the different devices indicated good agreement regarding
dominant species, overall diversity, and distribution of species among phylogenetic groups. Among the less common species,
there were few shared sequences in different clone libraries, likely due to the high diversity of the assessed samples. Bacteria
belonging to the Bacteroidetes and Proteobacteria phyla dominated at both locations, and the most common genera were Sphingomonas sp. and Pantoea sp. Chloroplast-like 16S rRNA gene sequences were detected in all samples. |
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