Lead Affects Apoptosis and Related Gene <Emphasis Type="Italic">XIAP</Emphasis> and <Emphasis Type="Italic">Smac</Emphasis> Expression in the Hippocampus of Developing Rats |
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Authors: | Junxiao Liu Dai Han Yan Li Liangkai Zheng Chengwu Gu Zhongxian Piao William W Au Xijin Xu Xia Huo |
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Affiliation: | (1) Analytical Cytology Laboratory and the Key Immunopathology Laboratory of Guangdong Province, Shantou University Medical College, 22 Xinling Road, 515041 Shantou, Guangdong, People’s Republic of China;(2) Department of Preventive Medicine, Shantou University Medical College, Shantou, People’s Republic of China; |
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Abstract: | Lead (Pb) exposure poses devastating effects on central nervous system development of children. To replicate aspects of this
neurotoxicity, we examined the effect of lead on the expression of apoptosis and of apoptosis-related genes, XIAP (X chromosome-linked
inhibitor of apoptosis protein) and Smac (second mitochondrial activator of caspase), in the hippocampus of developing rats.
A total of 48 rats (30-day old) were randomly divided into four groups for intragastrical perfusion of lead acetate Pb(Ac)2]: untreated, low (2 mg/kg/d), medium (20 mg/kg/d), and high (200 mg/kg/d) dose groups. Pb content was determined in blood,
and the apoptosis indexes and XIAP and Smac gene expression were analyzed in the hippocampus. There was a significant difference in apoptosis indexes (AI) between the
exposed and control groups (p < 0.01). AI was highest in the high exposure group. XIAP gene expression was reduced in the exposed groups and the expression was negatively correlated with blood lead levels (BLLs)
(p < 0.05). But the four groups did not differ in the expression of Smac (p > 0.05). Our data indicate that exposure to Pb(Ac)2 caused a dose-dependent and significant increase of apoptosis in the hippocampus of developing rats through depressing the
expression of the XIAP but not the Smac genes. |
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