The regulation of HanA during heterocyst development in cyanobacterium Anabaena sp. PCC 7120 |
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Authors: | Jing-jing Lu Lei Shi Wen-li Chen Li Wang |
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Institution: | 1. State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China 2. Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China 3. INRA, UMR1319 Micalis, 78350, Jouy-en-Josas, France
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Abstract: | In response to deprivation of combined nitrogen, the filamentous cyanobacterium Anabaena sp. strain PCC 7120 develops heterocyst, which is specifically involved in the nitrogen fixation. In this study, we focused on the regulation of HanA, a histone-like protein, in heterocyst development. Electrophoretic mobility shift assay results showed that NtcA, a global nitrogen regulator necessary for heterocyst differentiation, could bind to two NtcA-binding motifs in the hanA promoter region. qPCR results also showed that NtcA may regulate the expression of hanA. By using the hanA promoter-controlled gfp as a reporter gene and performing western blot we found that the amount of HanA in mature heterocysts was decreased gradually. |
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