Molecular characterization and gene expression of lhcb5 gene encoding CP26 in the light-harvesting complex II of Chlamydomonas reinhardtii |
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Authors: | Minagawa Jun Han Kab-Cho Dohmae Naoshi Takio Koji Inoue Yorinao |
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Institution: | (1) Laboratory for Photo-Biology, Photodynamic Research Center, Institute of Physical and Chemical Research (RIKEN), 519-1399 Aoba, Aramaki, Aoba, Sendai, Miyagi, 980-0845, Japan;(2) Department of Life Science, UIDUK University, Kyoung-Ju, KyungBuk, 780-910 , Korea;(3) Division of Biomolecular Characterization, Institute of Physical and Chemical Research (RIKEN), 2-1 Hirosawa, Wako, Saitama , 351-0198, Japan;(4) RIKEN Harima Institute at Spring-8, Kouto, Mikazuki, Sayo, Hyogo , 679-5148, Japan |
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Abstract: | We isolated and sequenced a cDNA clone encoding a minor chlorophyll a/b-binding protein, CP26, which is associated with the light-harvesting complex II of Chlamydomonas reinhardtii. Protein sequences of internal peptide fragments from purified CP26 were determined and used to identify a cDNA clone. The 1.1 kb lhcb5 gene codes for a polypeptide of 289 amino acids with a predicted molecular weight of 30713. The lhcb5 gene product could reconstitute with chlorophylls and xanthophylls to form a green band on a gel. Although the expression of many lhcb genes are strictly regulated by light, the lhcb5 gene was only loosely regulated. We propose that a plant acclimatizes itself to the light environment by quantitatively and qualitatively modulating the light-harvesting complex. Characterization of the primary structure and the implications of its unique expression are discussed. |
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Keywords: | cab Chlamydomonas reinhardtii CP26 lhcb light-harvesting complex |
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