Scale-up of hydrophobic interaction chromatography for the purification of a DNA vaccine against rabies |
| |
| Authors: | MM Diogo S Ribeiro JA Queiroz GA Monteiro P Perrin N Tordo DMF Prazeres |
| |
| Institution: | (1) Centro de Engenharia Biológica e Química, Instituto Superior Técnico, 1049-001 Lisboa, Portugal;(2) Departamento de Química, Universidade da Beira Interior, 6200 Covilhã, Portugal;(3) Laboratoire des Lyssavirus, Institute Pasteur, Paris, France |
| |
| Abstract: | The purification of a DNA vaccine against rabies by hydrophobic interaction chromatography (HIC) using a Sepharose gel derivatised with 1,4-butanediol diglycidyl ether was scaled up 60 times. The purification profile was not affected by increased loadings (up to 15 mg DNA) and a product with a consistent quality was obtained. Fourteen mg of plasmid with an HPLC purity of 100% were obtained in one run, corresponding to a 95% yield. |
| |
| Keywords: | DNA vaccines hydrophobic interaction chromatography plasmid DNA rabies |
| 本文献已被 SpringerLink 等数据库收录！ |
|
