DNA methylation changes in photoperiod-thermo-sensitive male sterile rice PA64S under two different conditions |
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Authors: | Xiaojun Chen Jihong HuHongyuan Zhang Yi Ding |
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Affiliation: | State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, People''s Republic of China |
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Abstract: | Epigenetic modification can occur at a high frequency in crop plants and might generate phenotypic variation without changes in DNA sequences. DNA methylation is an important epigenetic modification that may contribute to environmentally-induced phenotypic variations by regulating gene expression. Rice Photoperiod-Thermo-Sensitive Genic Male Sterile (PTGMS) lines can transform from sterility to fertility under lower temperatures and short-day (SD) conditions during anther development. So far, little is known about the DNA methylation variation of PTGMS throughout the genome in rice. In this study, we investigated DNA cytosine methylation alterations in the young panicles of PTGMS line PA64S under two different conditions using methylation sensitive amplified polymorphism (MSAP) method. Compared with the DNA methylation level of PA64S under lower temperatures and SD conditions (fertility), higher methylation was observed in PA64S (sterility). The sequences of 25 differentially amplified fragments were successfully obtained and annotated. Three methylated fragments, which are homologous to D2, NAD7 and psaA, were confirmed by bisulfite sequencing and their expression levels were also evaluated by qPCR. Real time quantitative PCR analysis revealed that five of the six selected methylated genes were downregulated in PA64S (sterility). These results suggested that DNA methylation may be involved in the sterility–fertility transition of PA64S under two different environmental conditions. |
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Keywords: | PTGMS, Photoperiod-Thermo-Sensitive Genic Male Sterile SD, short-day LD, long-day MSAP, methylation sensitive amplified polymorphism PA64S, Pei'ai 64S PA64S (S), PA64S (sterility) PA64S (F), PA64S (fertility) CMS, Cytoplasmic Male Sterile PSMS, photoperiod-sensitive genic male sterile LDMAR, long-day-specific male-fertility-associated RNA DM, differentially methylated psaA, photosystem I P700 chlorophyll A apoprotein A1 Cytf, cytochrome f D2, photosystem II protein D2 NAD7, NADH dehydrogenase subunit 7 VIP2, putative VIP2 protein TE, transposable element |
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