Characteristic of PGDS potential regulation role on spermatogenesis in the Chinese mitten crab Eriocheir sinensis |
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| Authors: | Di-An Fang Quan-Zhong Yang Jin-Rong Duan Qun Wang Min-Ying Zhang Yan-Feng Zhou Kai Liu Wei-Gang Shi |
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| Affiliation: | 1. Scientific Observing and Experimental Station of Fishery Resources and Environment in the Changjiang River, Freshwater Fisheries Research Center, Wuxi, Shanshui Road 9, 214081, China;2. Sanquan College, Xinxiang Medical University, Xinxiang, Henan 453003, China;3. School of Life Science, East China Normal University, Shanghai 200062, China |
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| Abstract: | Prostaglandin D synthase (PGDS) catalyzes the isomerization of PGH2 to produce PGD2 in the presence of sulfhydryl compounds. In this study, a full length PGDS gene comprising 1250 nucleotides from the Chinese mitten crab Eriocheir sinensis (Es-PGDS) was characterized, with a 615 bp open reading frame encoding 204 amino acid residues. Its deduced peptide has high homology with other species' PGDS protein. The Es-PGDS mRNA expression was tissue-related, with the highest expression observed in the hepatopancreas, accessory sex gland, testis and ovaries. We also detected the different stages of tissue expression and the enzyme activity for Es-PGDS in the testis and male crab hepatopancreas. The different expression patterns and its corresponding enzyme activity level indicated that PGDS is involving in the regulation of reproductive action during the period of rapid development in E. sinensis. Furthermore our research could arouse a heat debate on the PGDS reproductive function in invertebrate and further study will be needed to determine the molecular mechanism(s) linking PGDS functions to spermatogenesis and ontogenesis if this gene is to be exploited as a molecular biomarker in further studies of development. |
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| Keywords: | PGDS, prostaglandin D synthase GSH, glutathione PGs, prostaglandins PGD2, prostaglandin D2 COX, cyclooxygenase L-PGDS, lipocalin-type PGD synthase H-PGDS, hematopoietic PGD synthase Es-PGDS, E. sinensis PGDS gene sqPCR, semi-quantitative RT-PCR qPCR, real-time quantitative PCR RACE, rapid amplification of cDNA ends Gp, gene-specific primer NJ, neighbor-joining ISH, in situ hybridization OCT, optimal cutting temperature DEPC, diethylpyrocarbonate PBS, phosphate buffer saline DIG, digoxigenin NBT/BCIP, nitroblue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate H& E, hematoxylin&ndash eosin GST, glutathione S-transferase |
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