Bovine CLEC7A genetic variants and their association with seropositivity in Johne's disease ELISA |
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Authors: | SD Pant CP Verschoor FS Schenkel Q You DF Kelton NA Karrow |
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Institution: | 1. Centre for Genetic Improvement of Livestock, Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario N1G 2W1, Canada;2. Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1, Canada |
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Abstract: | Mycobacterium avium ssp. paratuberculosis (MAP) infection in cattle causes significant economic losses to the dairy and beef industries resulting from reduced productivity, premature culling and mortality. Bovine Dectin-1, an important pattern recognition molecule that is able to generate a proinflammatory response by acting alongside Toll like receptor (TLR) signaling, is known to co-operate with TLR2 to specifically activate a macrophage proinflammatory response against mycobacterial infections. Therefore, the goal of this study was to identify single nucleotide polymorphisms (SNPs) in the gene encoding bovine Dectin-1 (CLEC7A) and to assess their association with susceptibility to MAP infection in dairy cattle. Blood and milk samples, collected from commercial dairy operations, were tested for MAP infection using blood and milk ELISAs and a resource population consisting of 197 infected and 242 healthy cattle was constructed. Pooled DNA was used for sequencing and eight single nucleotide polymorphisms (SNPs) were identified. Identified SNPs were genotyped on the resource population using the iPLEX MassARRAY system and statistical analysis was performed using logistic regression fitting the additive and dominance effects of each SNP in the model. Out of a total of eight identified SNPs, five were successfully genotyped, and three out of these five SNPs were found to be in complete linkage. Statistical analysis revealed a strong association between a non-synonymous SNP c.589A>G (p = 0.008), and MAP infection status of the resource population inferred by seropositivity in MAP antibody specific ELISAs. This SNP c.589A>G was located in the geneic region that encodes the carbohydrate recognition domain of bovine Dectin-1. Therefore, further investigation of its functional relevance is warranted. |
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Keywords: | AIC Akaike's information criterion BTA Bos taurus autosome CD Crohn's disease CLEC7A C-type lectin domain family 7 member A/Dectin-1 CTL C-type lectin D Linkage disequilibrium ELISA Enzyme linked immuno sorbent assay IFNG Interferon-γ IL12 Interleukin-12 ITAM Immune receptor tyrosine-based activation motif JD Johne's disease MAP Mycobacterium avium ssp paratuberculosis NC Negative control OD Optical density OR Odds ratio PC Positive control PCR Polymerase chain reaction PRM Pattern recognition molecule QTL Quantitative trait loci S Sample SNP Single nucleotide polymorphism SP110 SP110 nuclear body protein Th1 T-helper 1 TLR Toll like receptor |
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