Identification of the glycerol kinase gene and its role in diapause embryo restart and early embryo development of Artemia sinica |
| |
| Authors: | Cheng Cheng Feng Yao Bing Chu Xuejie Li Yan Liu Yang Wu Yanli Mei Peisheng Wang Lin Hou Xiangyang Zou |
| |
| Institution: | 1. College of Life Sciences, Liaoning Normal University, Dalian 116081, PR China;2. Department of Biology, Dalian Medical University, Dalian 116044, PR China |
| |
| Abstract: | Glycerol kinase (GK) catalyzes the rate-limiting step in glycerol utilization by transferring a phosphate from ATP to glycerol, yielding glycerol 3-phosphate, which is an important intermediate for both energy metabolism and glycerolipid production. Artemia sinica has an unusual diapause process under stress conditions of high salinity, low temperature and lack of food. In the process, diapause embryos of A. sinica (brine shrimp) accumulate high concentrations of glycerol as a cryoprotectant to prevent low temperature damage to embryos. Upon embryo restart, glycerol is converted into glucose and other carbohydrates. Therefore, GK plays an important role in the diapause embryo restart process. However, the role of GK in diapause termination of embryo development in A. sinica remains unknown. |
| |
| Keywords: | GK glycerol kinase As-gk glycerol kinase gene of Artemia sinica G3P glycerol 3-phosphate FBP fructose-1 6-bisphosphate PTS phosphotransferase system ISH in situ hybridization NJ neighbor-joining DIG Digoxigenin PBS phosphate-buffered saline DEPC diethylpyrocarbonate RT-PCR real-time PCR LSD least square difference ORF open reading frame PI isoelectric point GKD glycerol kinase deficiency |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
