VdMsb regulates virulence and microsclerotia production in the fungal plant pathogen Verticillium dahliae |
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Authors: | Liangliang Tian Jun XuLei Zhou Wangzhen Guo |
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Affiliation: | State Key Laboratory of Crop Genetics & Germplasm Enhancement, Hybrid Cotton R&D Engineering Research Center, MOE, Nanjing Agricultural University, Nanjing 210095, China |
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Abstract: | The vascular wilt fungus Verticillium dahliae infects the roots of cotton plants and can seriously diminish the yield and quality of this and other dicotyledons. However, the key genes involved in V. dahliae infection and pathogenesis in cotton remain unclear. Msb encodes a transmembrane mucin that is highly conserved in the MAPK signal pathway. Msb has been implicated previously in pathogenicity in various aerial plant fungi. In this study, V. dahliae Msb (VdMsb) was found to be required for fungal virulence and microsclerotia production. Strains lacking VdMsb exhibited reduced conidiation and microsclerotia formation. Compared with wild-type and gene-complemented strains, the invasive growth and adhesive capacity of VdMsb deletion mutants were significantly decreased. These results suggest that VdMsb plays a role in development and virulence in V. dahliae. |
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Keywords: | MAPK, mitogen-activated protein kinase FG, Filamentous Growth HOG, high osmolarity glycerol PDA, potato dextrose agar CM, complete medium ATMT, Agrobacterium tumefaciens-mediated transformation ORF, Open Reading Frame TM, transmembrane region CT, C-terminus |
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