Creatine prevents the imbalance of redox homeostasis caused by homocysteine in skeletal muscle of rats |
| |
Authors: | Janaí na Kolling,Emilene B.S. SchererCassiana Siebert,Eduardo Peil MarquesTiago Marcom dos Santos,Angela T.S. Wyse |
| |
Affiliation: | 1. Laboratório de Neuroproteção e Doenças Neurometabólicas, Departamento de Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600-Anexo, CEP 90035-003, Porto Alegre, RS, Brazil;2. Laboratório de Erros Inatos do Metabolismo, Departamento de Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600-Anexo, CEP 90035-003, Porto Alegre, RS, Brazil |
| |
Abstract: | Homocystinuria is a neurometabolic disease caused by severe deficiency of cystathionine beta-synthase activity, resulting in severe hyperhomocysteinemia. Affected patients present several symptoms including a variable degree of motor dysfunction, being that the pathomechanism is not fully understood. In the present study we investigated the effect of chronic hyperhomocysteinemia on some parameters of oxidative stress, namely 2′7′dichlorofluorescein (DCFH) oxidation, levels of thiobarbituric acid-reactive substances (TBARS), antioxidant enzyme activities (SOD, CAT and GPx), reduced glutathione (GSH), total sulfhydryl and carbonyl content, as well as nitrite levels in soleus skeletal muscle of young rats subjected to model of severe hyperhomocysteinemia. We also evaluated the effect of creatine on biochemical alterations elicited by hyperhomocysteinemia. Wistar rats received daily subcutaneous injection of homocysteine (0.3–0.6 μmol/g body weight), and/or creatine (50 mg/kg body weight) from their 6th to the 28th days age. Controls and treated rats were decapitated at 12 h after the last injection. Chronic homocysteine administration increased 2′7′dichlorofluorescein (DCFH) oxidation, an index of production of reactive species and TBARS levels, an index of lipoperoxidation. Antioxidant enzyme activities, such as SOD and CAT were also increased, but GPx activity was not altered. The content of GSH, sulfhydril and carbonyl were decreased, as well as levels of nitrite. Creatine concurrent administration prevented some homocysteine effects probably by its antioxidant properties. Our data suggest that the oxidative insult elicited by chronic hyperhomocystenemia may provide insights into the mechanisms by which homocysteine exerts its effects on skeletal muscle function. Creatine prevents some alterations caused by homocysteine. |
| |
Keywords: | DCFH, dichlorodihydrofluorescein diacetate TBARS, thiobarbituric acid-reactive substances SOD, superoxide dismutase CAT, catalase GPx, glutathione peroxidase GSH, reduced glutathione Hcy, homocysteine HCU, homocystinuria CβS, cystathionine β-synthase RS, reactive species DNA, deoxyribonucleic acid RNA, ribonucleic acid NADPH, nicotinamide adenine dinucleotide phosphate O2&minus , superoxide anion NOS, nitric oxide synthase GAA, guanidinoacetic acid DTNB, 5,5&prime -dithio-bis (2-nitrobenzoic acid) EDTA, ethylenediamine tetraacetic acid ANOVA, one-way analysis of variance |
本文献已被 ScienceDirect 等数据库收录! |
|