Functional annotation of putative hypothetical proteins from Candida dubliniensis |
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Authors: | Kundan Kumar Amresh PrakashMunazzah Tasleem Asimul IslamFaizan Ahmad Md. Imtaiyaz Hassan |
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Affiliation: | Center for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi 110025, India |
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Abstract: | An extensive analysis of C. dubliniensis proteomics data showed that ~ 22% protein are conserved hypothetical proteins (HPs) whose function is still not determined precisely. Analysis of gene sequence of HPs provides a platform to establish sequence–function relationships to a more profound understanding of the molecular machinery of organisms at systems level. Here we have combined the latest versions of bioinformatics tools including, protein family, motifs, intrinsic features from the amino acid sequence, sequence–function relationship, pathway analysis, etc. to assign a precise function to HPs for which no any experimental information is available. Our results show that 27 HPs have well defined functions and we categorized them as enzyme, nucleic acid binding, transport protein, etc. Five HPs showed adhesin character that is likely to be essential for the survival of yeast and pathogenesis. We also addressed issues related to the sub-cellular localization and signal peptide identification which provides an idea about its colocalization and function. The outcome of the present study may facilitate better understanding of mechanism of virulence, drug resistance, pathogenesis, adaptability to host, tolerance for host immune response, and drug discovery for treatment of C. dubliniensis infections. |
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Keywords: | HP, hypothetical protein BLAST, basic local alignment search tool PSI-BLAST, position specific iterative basic local alignment search tool HMMTOP, prediction of transmembrane helices and topology of proteins TMHMM, membrane protein topology prediction method based on a hidden Markov model CATH, class, architecture, topology and homology GRAVY, grand average of hydropathicity CDD, Conserved Domain Database SMART, simple modular architecture research tool PANTHER, Protein ANalysis THrough Evolutionary Relationships SVM, Support Vector Machine PP2C, protein phosphatase 2C SAM, S­ adenosyl methionine DGK, diacylglycerol kinase CMD, carboxymuconolactone decarboxylase MFS, major facilitator superfamily |
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