A particular set of small non-coding RNAs is bound to the distinctive Argonaute protein of Trypanosoma cruzi: Insights from RNA-interference deficient organisms |
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Authors: | Maria Rosa Garcia-Silva,Julia Sanguinetti,Florencia Cabrera-Cabrera,Oscar Franzé n,Alfonso Cayota |
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Affiliation: | 1. Laboratory of Functional Genomics, Institut Pasteur of Montevideo, Montevideo, Uruguay;2. Icahn School of Medicine at Mount Sinai, Department of Genetics and Genomic Sciences, New York, NY, USA;3. Department of Medicine, Hospital de Clínicas, Montevideo, Uruguay |
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Abstract: | The study of small RNAs and Argonaute proteins in eukaryotes that are deficient in functional RNA interference could provide insights into novel functions of small RNAs. In this study we describe small non-coding RNAs bound to a distinctive Argonaute protein of Trypanosoma cruzi, TcPIWI-tryp. Co-immunoprecipitation of TcPIWI-tryp followed by deep sequencing of isolated RNA identified abundant small RNAs derived from rRNAs and tRNAs. The small RNA repertoire differed from that of the canonical Argonaute in organisms with functional RNA interference, which could indicate novel biological functions for TcPIWI-tryp in T. cruzi and other members of the trypanosomatid clade. |
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