Trp28Arg/Ile35Thr LHB gene variants are associated with elevated testosterone levels in women with polycystic ovary syndrome |
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Authors: | Mariani Carla Prudente Batista Eliane de Fatima Duarte Michele Delarmelina dos Reis Borba Emilie Zingler João Mangussi-Gomes Beatriz Taynara Araújo dos Santos Olivia Laquis de Moraes Sylvia Asaka Yamashita Hayashida Edmund C Baracat Francisco de Assis da Rocha Neves Gustavo Arantes Rosa Maciel Tania Aparecida Sartori Sanchez Bachega Gustavo Barcelos Barra Adriana Lofrano-Porto |
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Institution: | 1. Ambulatório de Endocrinologia das Gônadas e Adrenais, Unidade de Endocrinologia, Hospital Universitário de Brasília (HUB), Universidade de Brasília (UnB), Brazil;2. Unidade de Endocrinologia, Hospital Regional de Taguatinga, Brasília, DF, Brazil;3. Departmento de Psiquiatria, HUB, UnB, Brazil;4. Department of Family Medicine, McGill University, Montreal, Canada;5. Laboratório de Farmacologia Molecular, Faculdade de Ciências da Saúde, UnB, Brazil;6. Disciplina de Ginecologia, Faculdade de Medicina da Universidade de São Paulo (FMUSP), São Paulo, Brazil;g Laboratório de Hormônios e Genética Molecular — LIM 42, FMUSP, Brazil;h Unidade de Biologia Molecular, Laboratório e Instituto Sabin, Brasilia, DF, Brazil |
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Abstract: | IntroductionPolycystic Ovary Syndrome (PCOS) is a complex endocrine disorder, of multifactorial etiology, which affects 6–10% of women of reproductive age. It is considered the leading cause of anovulatory infertility, menstrual disorders and hyperandrogenism in this population. The genetic basis of PCOS is still largely unknown despite significant family clustering; determining its mode of inheritance is particularly difficult given the heterogenic presentation of the disease.Materials and methods130 Brazilian women, aged 14–42 years, who met the 2003 Rotterdam criteria for PCOS diagnosis, were included, and 96 healthy women constituted the control group. Presence of hirsutism was classified using the modified Ferriman–Gallwey score (F–G score) as absent (≤ 7), mild (8–14), and severe (≥ 15). Blood levels of luteinizing hormone (LH), total testosterone (TT), dehydroepiandrosterone sulfate (DHEA-S) and androstenedione were determined. The coding region of the luteinizing hormone beta-subunit (LHB) gene was amplified and sequenced. Differences in allelic and genotypic frequency distribution of each polymorphism across controls and cases were estimated by the Mantel–Haenszel chi-square or Fisher's exact test (p < 0.05), and the probability of an association between the detection of a polymorphism and presence of a diagnosis of PCOS, by logistic regression.Result(s)Sequencing detected 8 polymorphisms in the LHB gene coding region. Two polymorphisms in linkage disequilibrium were significantly more prevalent in the presence of hyperandrogenemia: rs1800447/rs34349826 (Trp28Arg/Ile35Thr) (p = 0.02).Conclusion(s)In this series, a modulatory effect of LHB polymorphisms on hyperandrogenemia phenotype of PCOS was observed; however, this finding needs to be replicated in other populations. |
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Keywords: | LH luteinizing hormone LHB luteinizing hormone beta subunit PCOS Polycystic Ovary Syndrome BMI body mass index TT total testosterone DHEA-S dehydroepiandrosterone sulfate IFG impaired fasting glucose levels F&ndash G score Ferriman&ndash Gallwey score GWAS genome-wide association study AR androgen receptor |
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