Polymorphism analysis in genes of the somatotropic axis in Nellore cattle selected for growth |
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| Authors: | Diercles Francisco Cardoso,Fá bio Ricardo Pablos de Souza,Gregó rio Miguel Ferreira de Camargo,Patrí cia Dias da Silva Fonseca,Larissa Fernanda Simielli Fonseca,Camila Urbano Braz,Arione Augusti Boligon,Maria Eugê nia Zerlotti Mercadante,Lucia Galvã o de Albuquerque,Humberto Tonhati |
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| Affiliation: | 1. São Paulo State University, Jaboticabal, SP, Brazil;2. Instituto de Zootecnia, Centro APTA Bovinos de Corte, Sertãozinho, SP, Brazil |
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| Abstract: | The somatotropic axis consists of genes that are involved in muscular development. These genes are potential regions of study to identify possible QTL for economically important traits in beef cattle. The aim of this study was to verify the existence of GH1, POU1F1, and GHR polymorphisms in Nellore cattle to verify the influence of selection in these mutations and to analyse the association between molecular markers and body weight at different ages, yearling hip height, carcass fat thickness and loin eye area. Six hundred forty-five animals from the Centro APTA Bovinos de Corte, were genotyped by PCR–RFLP techniques. The association analyses were performed with general mixed models taking into consideration the effect of one marker, and other model taking into consideration interactions between two molecular markers. Only the molecular markers rs81109601 on GH1 and rs109136815 on GHR were polymorphic; however, they were not found to be under selection. The association of the GHR rs109136815 marker and loin eye area was observed (p < 0.05), as well as the effect of interaction between the markers and the female body weight at 550 days of age (p < 0.04). The interaction effect should be considered in situations where the interactivity between two genes is known. |
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| Keywords: | A>G, substitution adenosine by guanosine BF, back fat thickness BW, body weight C>A, substitution of cytidine by adenosine GH, growth hormone protein GH1, growth hormone gene GHR, growth hormone receptor gene H378, hip height measured in 378 days H550, hip height measured in 550 days LMA, loin eye area of longissimus muscle NeC, line of selection named Nellore control NeS, line of selection named Nellore selection NeT, line of selection named Nellore traditional POU1F1, POU class 1 homeobox 1 gene QTL, quantitative trait loci RF, rump fat thickness T>C, substitution of thymidine by cytidine W210, weaning body adjust to 210 days W378, weaning body adjust to 378 days W550, weaning body adjust to 550 days |
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