Associations of variants in MTHFR and MTRR genes with male infertility in the Jordanian population |
| |
Authors: | Doaa S. Mfady May F. Sadiq Omar F. Khabour AbdulFattah S. Fararjeh Aymen Abu-Awad Yousef Khader |
| |
Affiliation: | 1. Department of Biological Sciences, Faculty of Science, Yarmouk University, Irbid 22110, Jordan;2. Department of Medical Laboratory Sciences, Jordan University of Science and Technology, Irbid 22110, Jordan;3. AL-Hussein Medical City Hospital, Amman, Jordan.;4. Department of Public Health, Jordan University of Science and Technology, Irbid 22110, Jordan |
| |
Abstract: | Folate pathway is expected to play an important role in spermatogenesis since it is involved in DNA synthesis, repair and methylation. The purpose of this study was to examine the association between male infertility and the MTHFR (C677T and A1298C) and MTRR (A66G) polymorphisms. A group of 300 males was recruited in this study from different Jordanian infertility clinics. Of these, 150 cases of infertile men that included oligozoospermia cases (n = 45), severe oligozoospermia (n = 71) and azoospermia (n = 34) were studied. The other 150 males were age matched fertile controls. Genotyping of MTHFR and MTRR polymorphisms was performed using PCR-RFLP technique. The results showed an association between MTHFR 677TT genotype and male infertility (P < 0.05). However, the distribution of MTHFR A1298C and MTRR A66G genotypes were not different between the fertile and infertile groups (P > 0.05). In addition, none of the examined polymorphisms was related to any of the semen parameters in the infertile group. In conclusion, this study showed that MTHFR C677T polymorphism is associated with male infertility in Jordanians. |
| |
Keywords: | CI, confidence interval DNA, deoxyribonucleic acid EDTA, ethylene diamine tetra-acetate IVF, in vitro facilitated MTHFR, methylene tetrahydrofolatereductase MTRR, methionine synthase reductase OR, odd ratio PCR, polymerase chain reaction RFLP, restriction fragment length Polymorphism SNP, single nucleotide polymorphism |
本文献已被 ScienceDirect 等数据库收录! |
|