A rapid method for the reconstitution of Na+-dependent neutral amino acid transport from bovine renal brush-border membranes. |
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| Authors: | A M Lynch and J D McGivan |
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| Institution: | Department of Biochemistry, Medical School, Bristol, U.K. |
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| Abstract: | 1. A simple and rapid method for the reconstitution of Na+-dependent neutral amino acid transport activity from bovine renal brush border membranes is described. 2. The neutral detergent decanoyl-N-methylglucamide ('MEGA-10') was employed to solubilize the membrane protein. This obviated the necessity for a prolonged dialysis step. 3. The properties of amino acid transport in these vesicles were similar to those observed in native membranes. 4. This should be a useful procedure in the eventual identification and isolation of amino acid transport proteins. |
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