Effect of puromycin on synthesis, processing, and nucleocytoplasmic translocation of rRNA in Tetrahymena pyriformis. |
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Authors: | W A Eckert |
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Institution: | Department of Physiology, University of Heidelberg, West Germany. |
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Abstract: | 1. Treatment of Tetrahymena pyriformis with various concentrations of puromycin results in a more pronounced inhibition of 3H]uridine accumulation in stable RNA than of protein synthesis. 2. At a concentration of 500 micrograms/ml, which is almost completely inhibitory to 3H]uridine incorporation in vivo, puromycin has no influence on the incorporation of 3H]UTP into RNA in isolated macronuclei. Pretreatment of the cells with the antibiotic, however, reduces the activity of RNA polymerases in isolated nuclei to less than 30%. 3. In puromycin-treated cells a small amount of pre-rRNA is synthesized but not processed into cytoplasmic rRNAs. 4. Puromycin reduces the nucleocytoplasmic translocation of pre-existing RNA to about 25% of the control rate within 5 min, resulting in an accumulation of relatively stable rRNA precursor molecules in the macronucleus. |
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