Massive excystation of Toxoplasma gondii sporozoites |
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Authors: | Freyre Alvaro Falcón Jesús |
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Institution: | Laboratory for Toxoplasmosis, Department for Parasitology, College for Veterinary Sciences, Alberto Lasplaces 1550, Montevideo, Uruguay. freyrealvaro@hotmail.com |
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Abstract: | In the present study, several stimuli were tested to liberate Toxoplasma gondii sporozoites from oocysts. Incubation in Na(2)CO(3)-CO(2) buffer at 40 degrees C with ultrasound, followed by incubation at 40 degrees C with bile or deoxycholic acid, was the only method that resulted in 95% liberation of motile infective sporozoites. A protocol of reproducibly high efficiency is provided, that compares favorably with any of the methods previously published. |
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