Well-identifiable human chromosomes Isolated from mitotic fibroblasts by a new method |
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| Authors: | Charles H C M Buys Trijntje Koerts Jacob A Aten |
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| Institution: | (1) Department of Human Genetics, State University of Groningen, Ant. Deusinglaan 4, 9713 AW Groningen, The Netherlands;(2) Laboratory of Radiobiology, University of Amsterdam, Plesmanlaan 121, NL-1066 CX Amsterdam |
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| Abstract: | Summary Metaphase chromosomes isolated from human fibroblasts by lysis of mitotic cells in the presence of the intercalating DNA-specific fluorochrome propidium iodide appear relatively long, even after exposure to vinblastine sulfate overnight. Therefore, they can be easily banded and thereby unequivocally identified. Chromosomes isolated in this way may be employed in flow analysis and sorting without loosing the inducibility of their band patterns. |
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