Acetyl esterase production by Termitomyces clypeatus |
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Authors: | A Mukhopadhyay PP Hazra T Sengupta AK Ghosh S Sengupta |
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Institution: | (1) Chemical Biology, Indian Institute, 4 Raja S.C. Mullick Road, Calcutta, 700 032, INDIA |
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Abstract: | Production of acetyl esterase by Termitomyces clypeatus was stimulated by xylan, cellulose, arabinose and arabinose-containing polysaccharides in the growth medium. The culture filtrate was equally active with p-nitrophenyl acetate and acetyl xylan. Acetyl xylan was completely deacetylated by the enzyme. Activity was optimum at pH 6.5 and at 50¡C. The Km values for p-nitrophenyl acetate and acetyl xylan were 0.83 mM and 0.38% (w/v) with Vm of 48 and 55 mmole acetate produced/min.mg protein, respectively. |
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