| 用PCR检测临床血标本中人类细小病毒B19 DNA |
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| 引用本文： | 杨洪江,张桦.用PCR检测临床血标本中人类细小病毒B19 DNA[J].病毒学报,1995,11(4):377-380. |
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| 作者姓名： | 杨洪江 张桦 |
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| 作者单位： | 天津市儿童医院儿研所 |
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| 摘 要： | 用ＰＣＲ检测临床血标本中人类细小病毒Ｂ１９ ＤＮＡ杨洪江，张桦，林书祥，牛艾茹（天津市儿童医院儿研所，天津３０００７４）关键词聚合酶链反应（ＰＣＲ），人类细小病毒Ｂ１９几种人类细小病毒中，只有Ｂ１９病毒是人类的病原体。由于该病毒不能在体外繁殖，获得该...
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| 关 键 词： | 聚合酶链反应 人类细小病毒 DNA 临床检验 |
POLYMERASE CHAIN REACTION ASSAY OF HUMANPARVOVIRUS B19 DNA IN CLINICAL BLOOD SPECIMENS |
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| Yang Hongjiang Zhang Hua Lin Shuxiang Niu Airu.POLYMERASE CHAIN REACTION ASSAY OF HUMANPARVOVIRUS B19 DNA IN CLINICAL BLOOD SPECIMENS[J].Chinese Journal of Virology,1995,11(4):377-380. |
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| Authors: | Yang Hongjiang Zhang Hua Lin Shuxiang Niu Airu |
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| Abstract: | he polymerase reaction(PCR)was used to detect human parvovirus B19 DNA ina panel of sera from hospitalized children.Small portion of each 100 serum samples weremixed,heat inactivated,and tested.The positive pools of each 100 mixed serum samples wererestested in pools of 10 and finally identification of the individual positive sample was carriedout by PCR amplification of 10 separately.Of 1 300 serum specimensanalyzed,8(0.6%)were found positive by the sequential PCR.The result indicates thatparvovirus B19 infection exists in Chinese children.This method has proven to reliable anduseful for large-scale screening of clinical specimens and for epidemiological survey. |
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| Keywords: | Polymerase chain reaction Human parvovirus B19 |
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