Functional activities of monomeric and dimeric forms of the chloroplast cytochrome b6f complex |
| |
Authors: | R K Chain R Malkin |
| |
Institution: | (1) Department of Plant Biology, University of California Berkeley, 111 Koshland Hall, 94720-3102, CA, USA |
| |
Abstract: | A monomeric form of the isolated cytochrome b6f complex from spinach chloroplast membranes has been isolated after treatment of the dimeric complex with varying concentrations of Triton X-100. The two forms of the complex are similar as regards electron transfer components and subunit composition. In contrast to a previous report (Huang et al. (1994) Biochemistry 33: 4401–4409) both the monomer and dimer are enzymatically active. However, after incorporation of the respective complexes into phospholipid vesicles, only the dimeric form of the cytochrome complex shows uncoupler sensitive electron transport, an indication of coupling of electron transport to proton translocation. The absence of this activity with the monomeric form of the cytochrome complex may be related to an inhibition by added lipids.Abbreviations CCCP-
carbonyl cyanide m-chlorophenylhydrazone
- mega-9-
nonanoyl-N-methylglucamide |
| |
Keywords: | cytochrome b6 cytochrome f plastoquinol-plastocyanin oxidoreductase dimer-monomer conversion liposomes |
本文献已被 SpringerLink 等数据库收录! |
|