Osmotic stress sensing in Populus: components identification of a phosphorelay system |
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Authors: | Chefdor Françoise Bénédetti Hélène Depierreux Christiane Delmotte Francis Morabito Domenico Carpin Sabine |
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Institution: | Laboratoire de Biologie des Ligneux et des Grandes Cultures (UPRES EA 1207), Faculté des Sciences, Université d'orleans, Rue de Chartres, BP6759, 45067 Orléans Cedex 2, France. |
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Abstract: | To study the Populus response to an osmotic stress, we have isolated one cDNA encoding a histidine-aspartate kinase (HK1) and four cDNAs encoding histidine-containing phosphotransfer proteins (HPts), HPt1-4. The predicted HK1 protein shares a typical structure with ATHK1 and SLN1 osmosensors. The 4 HPTs are characterized by the histidine phosphotransfer domain. We have shown that HK1 is upregulated during an osmotic stress in hydroponic culture. We have detected an interaction between HK1 and HPt2, using the yeast two-hybrid system. These results suggest the existence of a multi-step phosphorelay pathway probably involved in osmotic stress sensing in Populus. |
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Keywords: | Histidine-aspartate kinase Histidine-containing phosphotransfer protein Yeast two-hybrid analysis Osmotic stress Populus |
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