Localization of ribulose 1,5-bisphosphate carboxylase/oxygenase in the N2-fixing cyanobacterium Anabaena cylindrica

Abstract A new, high copy number conjugative plasmid pSLG3 (10.9 kb) was isolated from vegetative mycelium of Streptomyces lavendulae-grasserius RIA746. The sensitivity of pSLG3 DNA to 9 restriction endonucleases was tested and the positions of the unique Bgl II and Pst I target site, 3 Kpn I target sites and 5 Pvu II target sites were mapped. The unique Bgl II target site was localized outside pSLG3 essential region and was used for the construction of recombinant plasmid pSR1, composed of pSLG3 and pIJ350 Streptomyces DNAs.