Lippia dulcis shoot cultures as a source of the sweet sesquiterpene hernandulcin |
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| Authors: | Martina Sauerwein Hector E. Flores Takeshi Yamazaki Koichiro Shimomura |
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| Affiliation: | (1) Tsukuba Medicinal Plant Research Station, National Institute of Hygienic Sciences, 1 Hachimandai, Tsukuba, 305 Ibaraki, Japan;(2) Department of Plant Pathology and Biotechnology, Institute, Pennsylvania State University, 16802 University Park, PA, USA;(3) Division of Food Additives, National Institute of Hygienic Sciences, 1-18-1, Kamiyoga, Setagaya-ku, 158 Tokyo, Japan |
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| Abstract: | The axenic shoot culture of Lippia dulcis Trev., Verbenaceae, was established on hormone-free Murashige-Skoog solid medium containing 3% sucrose. Shoots were cultured in various liquid or solid media. Woody Plant liquid medium was best for shoot multiplication, but the production of hernandulcin was relatively low. The highest hernandulcin content (2.9% dry wt) was obtained after 28 days of culture on Murashige-Skoog solid medium containing 2% sucrose. The addition of chitosan to the culture media enhanced the growth of shoots as well as the production of hernandulcin, especially with the liquid medium.Abbreviations MS(2%) Murashige-Skoog medium containing 2 % sucrose - MS(3%) Murashige-Skoog medium containing 3 % sucrose - 1/2MS half strength Murashige-Skoog medium containing 2% sucrose - B5 Gamborg B5 medium containing 2% sucrose - WP Woody Plant medium containing 2% sucrose |
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| Keywords: | Lippia dulcis Shoot culture Chitosan Hernandulcin |
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