十字花科蔬菜基因组含量的测定与分析

以我国14种重要十字花科蔬菜为材料,利用流式细胞术测定基因组合量.其中青苤蓝、乌塌菜、雪里蕻、芥蓝的基因组含量属首次报道.本试验数据与国外文献提供的相关数据对比,发现青萝卜、结球甘蓝、青花菜和根用芥菜的基因组含量与已报道数值基本吻合,而大白菜、花椰菜的基因组含量值与报道数据存在差异.造成同一物种基因组含量值差异的因为可能是品种的不同,也可能与生长环境或测定时参考标准选用等因素不同有关.

Analysis of Genome Contents in Some Cruciferous Vegetables

The genome contents of 14 major cruciferous vegetables were measured. The genome contents of green-kohlrabi (Brassica oleracea L. var. caulorapa D.C.) , savoy (Brassica Chinensis var. rosularis Tsen et Lee.) , leaf mustard (Brassica juncea var. multiceps Tsen et Lee.) and Chinese kale (Brassica alboglabra Bailey) were firstly reported in the current study. Comparing the relevant data and numerical of foreign literature provided, the genome contents of the green radish (Raphanus sativus Bailey) , cabbage (Brassica oleracea L. var. capitata) , broccoli (Brassica. oleracea L. var. italica) and root mustard (Brassica juncea Coss) were basically consistent with the reported values, the genome contents of the heading Chinese cabbage (Brassica campestris L. var. cephalata Tsen et Lee) and cauliflower (Brassica oleracea L .var .botrytis L.) were some different from the reported data. The reasons which cause the variation in genome content of the same species were possibly the difference of breed, the diverse growing environment or the inconsistent reference standard.