IRS-PCR-based genetic mapping of the huntingtin interacting protein gene (HIP1) on mouse Chromosome 5 |
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Authors: | Heinz Himmelbauer Niels Wedemeyer Thomas Haaf Erich E Wanker Leonard C Schalkwyk Hans Lehrach |
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Institution: | (1) Max-Planck-Institute for Molecular Genetics, Ihnestr. 73, D14195 Berlin-Dahlem, Germany, DE |
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Abstract: | Huntington's disease (HD) is a devastating central nervous system disorder. Even though the gene responsible has been positionally
cloned recently, its etiology has remained largely unclear. To investigate potential disease mechanisms, we conducted a search
for binding partners of the HD-protein huntingtin. With the yeast two-hybrid system, one such interacting factor, the huntingtin
interacting protein-1 (HIP-1), was identified (Wanker et al. 1997; Kalchman et al. 1997) and the human gene mapped to 7q11.2.
In this paper we demonstrate the localization of the HIP1 mouse homologue (Hip1) into a previously identified region of human-mouse synteny on distal mouse Chromosome (Chr) 5, both employing an IRS-PCR-based
mapping strategy and traditional fluorescent in situ hybridization (FISH) mapping.
Received: 7 August 1997 / Accepted: 5 September 1997 |
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