| 农杆菌介导将白叶枯病抗性基因Xa21导入水稻获得转基因植株 |
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| 引用本文： | 姚方印,李中华,等.农杆菌介导将白叶枯病抗性基因Xa21导入水稻获得转基因植株[J].西北植物学报,2002,22(5):1038-1043. |
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| 作者姓名： | 姚方印 李中华 |
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| 作者单位： | 1. 山东省水稻研究所,山东,济宁,272017
2. 济宁市种子公司,山东,济宁,272037 |
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| 基金项目： | 国家转基因植物研究与产业化专项资助 (J99-B-0 1 8)，山东省青年基金资助项目 (Y2 0 0 0 D0 9) |
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| 摘 要： | 利用农杆菌介导的高效遗传转化系统，将白叶枯病抗性基因Xa21转入黄淮稻区主栽品种豫粳6号的胚性愈伤组织，获得转基因植株，GUS染色和PCR分析证明Xa21基因已整合到水稻基因组中，其自交T1代植株经GUS染色和白叶枯病接种鉴定呈现3：1分离，研究为培育抗白叶枯病水稻品种奠定了基础。
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| 关 键 词： | 农杆菌介导 白叶枯病抗性基因Xa21 水稻 转基因植株 |
| 文章编号： | 1000-4025(2002)05-1038-06 |
| 修稿时间： | 2001年10月26 |
Regeneration of transgenic rice plants after trans-formation of a bacterial blight resistance gene Xa21 by using Agrobacterium-mediated system |
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| YAO Fang yin,LI Guang xian,YANG Lei,LI Zhong hu,ZHAI Hong mei,YANG Hai xu.Regeneration of transgenic rice plants after trans-formation of a bacterial blight resistance gene Xa21 by using Agrobacterium-mediated system[J].Acta Botanica Boreali-Occidentalia Sinica,2002,22(5):1038-1043. |
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| Authors: | YAO Fang yin LI Guang xian YANG Lei LI Zhong hu ZHAI Hong mei YANG Hai xu |
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| Institution: | YAO Fang yin 1,LI Guang xian 1,YANG Lei 1,LI Zhong hua 2,ZHAI Hong mei 2,YANG Hai xu 2 |
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| Abstract: | Using a high efficient rice transformation system mediated by Agrobacterium tumefaciens ,a bacterial blight resistance gene Xa 21 was introduced into calli of a japonica variety Yujing 6 cultivated in Huanghuai area,and transgenic plants were obtained. GUS staining and PCR analysis demonstrated that Xa 21 had been integrated into rice genome DNA.Based on GUS detection and inoculation identification,the inheritance of Xa 21 gene in T 1 progenies was basically accorded to a segregation ratio of 3:1.This experiment provides a basis for further resistant breeding to bacteria blight diseases. |
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| Keywords: | transgenic rice Xa 21 gene Agrobacterium Xanthomonas oryzae pv oryzae |
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