Cloning and chromosomal mapping of the mouse DNA-dependent protein kinase gene |
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Authors: | Kiyohiro Hamatani Yoichi Matsuda Ryoko Araki Masahiro Itoh M Abe |
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Institution: | (1) Cell Biology Laboratory, Department of Radiobiology, Radiation Effects Research Foundation, 8-6 Nakagawa 1 chome, Nagasaki 850, Japan, JP;(2) Laboratory of Animal Genetics, Nagoya University School of Agricultural Sciences, Furou-cho, Chigusa-ku, Nagoya 464-01, Japan, JP;(3) Division of Biology and Oncology, National Institute of Radiological Sciences, 4-9-1 Anagawa, Chiba 263, Japan, JP |
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Abstract: | Severe combined immune deficiency (scid) mice are assumed to have two types of abnormalities: one is high radiosensitivity and the other is abnormal recombination
in immunoglobulin and T-cell receptor genes. The human chromosome 8 q1.1 region has an ability to complement the scid aberrations. Moreover, the localization of the subunit DNA-dependent protein kinase DNA-PKcs] participating in DNA double-strand break repair in the same locus was clarified. In scid mouse cells, the number of DNA-PKcs products and extent of DNA-PK activity remarkably decrease. These observations gave rise to the assumption that DNA-PKcs is the scid factor itself. In order to determine whether the DNA-PK
cs
gene is the scid gene, we isolated the mouse DNA-PK
cs
gene and investigated its chromosomal locus by fluorescence in situ hybridization (FISH). Consequently, it became clear that
the mouse DNA-PK
cs
gene existed in the centromeric region of mouse chromosome 16, determined by cross-genetic study, as a scid locus. This finding strongly suggests that mouse DNA-PK
cs
is the scid gene.
Received: 22 March 1996 |
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Keywords: | |
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