Growth and viability of mycelial fragments of white-rot fungi on some hydrogels |
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| Authors: | D Leštan M Leštan R T Lamar |
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| Institution: | (1) Institute for Microbial and Biochemical Technology, USDA Forest Service, Forest Products Laboratory, One Gifford Pinchot Drive, Madison, WI 53705-2398, USA, US;(2) Center for Soil and Environmental Science, University of Ljubljana, Biotechnical Faculty, Agronomy Department, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia, SI;(3) Teslova ulica 6, SI-1000 Ljubljana, Slovenia, SI;(4) Intech 180 Corp, 1770 North Research Park Way, Suite 100, North Logan, UT 84341, USA, US |
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| Abstract: | The viability of mycelial fragments of Trametes versicolor and Irpex lacteus and their growth on selected hydrogels are described. The size of mycelial fragments of the fungi did not significantly influence
their viability. Alginate hydrogel films supported fungal growth better than agarose, carrageenan, chitosan and gelatin films,
and had the highest mechanical strength but were less hydrophilic than the other hydrogels. All commercial alginates that
were tested supported aseptic growth of fungal fragments without prior sterilization of the hydrogel solution. The viability
of mycelial fragments in the hydrogel solutions was higher for some commercial alginates than that in laboratory grade alginate.
The mechanical strength and hydrophilicity of hydrogels from alginate type Sobalg FD 155 and Meer HV were comparable to that
of laboratory grade alginate. Sterilization and pH of the alginate hydrogel did not significantly influence the growth of
T. versicolor mycelial fragments but affected the growth of I. lacteus. Concentrations of alginate in the range of 1–2% in the hydrogel did not affect the growth of entrapped mycelial fragments
of these fungi.
Received 25 June 1997/ Accepted in revised form 07 March 1998 |
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| Keywords: | : fungal inocula mycelial fragments commercial hydrogels |
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