Dibutyryl c-AMP as an inducer of sporidia formation: Biochemical and antigenic changes during morphological differentiation of Karnal bunt (<Emphasis Type="Italic">Tilletia indica</Emphasis>) pathogen in axenic culture |
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Authors: | Email author" target="_blank">Anil?KumarEmail author Kaushlendra?Tripathi Manish?Rana Shalini?Purwar G?K?Garg |
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Institution: | (1) Department of Molecular Biology and Genetic Engineering, GB Pant University of Agriculture and Technology, 263 145 Pantnagar, India |
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Abstract: | Effect of dibutyryl adenosine 3′,5′-cyclic monophosphate (dbc-AMP), an analogue of c-AMP, was investigated on growth and morphological
differentiation ofTilletia indica. Exponential growth was observed up to 21 days in both presence and absence of dbc-AMP; however, increasing concentration
of dbc-AMP was deleterious to mycelial growth in liquid culture. A slow increase of mycelial biomass up to 21 days and decline
at 30 days in the presence of 2.5 mM dbc-AMP was observed, therefore, this concentration was chosen in subsequent investigations.
The inhibitory influence of dbc-AMP was further substantiated by decrease in soluble protein. The fungus on exposure to dbc-AMP
experienced morphological differentiation from vegetative mycelial phase to sporogenous mycelial phase, and was induced to
produce filiform sporidia. Use of quantitative ELISA further suggested that sporidia formation took more than 21 days in the
presence of dbc-AMP. Variations of proteins during different stages ofT. indica grown in the presence and absence of dbc-AMP suggested the expression of stage-specific proteins or differential expression
of proteins induced by dbc-AMP. The changes in expression of cell surface antigens as evidenced from decrease and increase
binding of anti-mycelial and anti-sporidial antibodies in dbc-AMP treated culture by ELISA was further interpreted on the
basis of morphological differentiation from mycelial to sporidial phase |
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Keywords: | Antigenic properties axenic culture dibutyryl c-AMP ELISA Karnal bunt morphological differentiation sporidia Tilletia indica |
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