| 一株反硝化光合细菌的生物学特性及系统发育分析 |
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| 引用本文： | 陈慧,张德民,王龙刚,潘志崇. 一株反硝化光合细菌的生物学特性及系统发育分析[J]. 微生物学报, 2011, 51(2): 249-255 |
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| 作者姓名： | 陈慧 张德民 王龙刚 潘志崇 |
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| 作者单位： | 宁波大学,应用海洋生物技术教育部重点实验室,宁波,315211 |
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| 基金项目： | 教育部科学技术研究重点项目（208053）；教育部创新团队（IRT0734）；国家“863计划”（2007AA10Z409）；浙江省自然科学基金人才培养项目（R305333）；宁波市科技创新项目（2004A610026，2007A31004） |
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| 摘 要： | 【目的】养殖水体中亚硝酸盐的过量积累会对养殖生物产生毒害作用,应用脱氮细菌去除亚硝酸盐是养殖水质调控的重要手段之一,本文意在得到一株高效去除亚硝酸盐的光合细菌。【方法】采用软琼脂稀释法分离纯化光合细菌菌株,通过电镜观察、生理生化试验研究其生物学特性、依据16S rDNA和光合反应中心M亚基基因(Gene coding for photosynthetic reaction center subunit M,pufM)序列对其做系统发育分析。【结果】从淡水养殖塘中分离筛选到一株高效还原亚硝氮的光合细菌菌株wps。该菌株为革兰氏阴性菌,细胞杆状,大小为0.4-0.6μm×1.5-4.0μm,极生丛生鞭毛,片层状光合内膜,兼性厌氧光照条件生长,单菌落及液体培养物呈红色,含细菌叶绿素a和类胡萝卜素。最适生长pH范围为5.5-8.5,最适生长盐度范围为0-2%,最适生长温度范围为25℃-38℃。菌株wps与Rhodopseudomonas palustris的16S rDNA序列相似性为98.9%,光合反应中心M亚基基因序列的相似性为94.9%,但是二者在生物学性质上有较大差异,如菌株wps在pH5.5生长,不能光自养生长,不利用柠檬酸盐、甲酸盐进行光异养生长,需盐酸硫胺素和泛酸钙做生长因子等。【结论】菌株wps可能为Rhodopseudomonas属的一个新种,且在养殖水体水质调控中具有重要应用前景。
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| 关 键 词： | 光合细菌 红假单胞菌 亚硝酸盐 反硝化 |
| 收稿时间： | 2010-07-06 |
| 修稿时间： | 2010-10-25 |
Biological characteristics and phylogenetic analysis of a denitrifying photosynthetic bacterium |
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| Hui Chen,Demin Zhang,Longgang Wang and Zhichong Pan. Biological characteristics and phylogenetic analysis of a denitrifying photosynthetic bacterium[J]. Acta microbiologica Sinica, 2011, 51(2): 249-255 |
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| Authors: | Hui Chen Demin Zhang Longgang Wang Zhichong Pan |
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| Affiliation: | Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo 315211, China;Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo 315211, China;Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo 315211, China;Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education, Ningbo 315211, China |
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| Abstract: | [Objective]Nitrite accumulation in aquaculture water is toxic to reared animals.One of the solutions to this problem is to apply denitrifying bacteria.This paper is intended to get a strain of phototrophic bacteria for efficient removal of nitrite from aquaculture water.[Methods]We used soft agar to isolate and purify phototrophic bacteria.We investigated biological characteristics of the isolate by means of light and electronic observations,physical and chemical tests.We analyzed its phylogenetical positio... |
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| Keywords: | Keywords: photosynthetic bacteria Rhodopseudomonas nitrite denitrification |
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