Degradation of PCB congeners by bacterial strains |
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Authors: | Arno Rein Margit M Fernqvist Philipp Mayer Stefan Trapp Martin Bittens Ulrich Gosewinkel Karlson |
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Institution: | (1) Helmholtz Centre for Environmental Research - UFZ, Permoserstr. 15, 04318 Leipzig, Germany;(2) National Environmental Research Institute, University of Aarhus, P.O. Box 358, 4000 Roskilde, Denmark;(3) Institute of Environment and Resources, Technical University of Denmark, Bygningstorvet 115, 2800 Kongens Lyngby, Denmark |
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Abstract: | Biological in situ methods are options for the remediation of contaminated sites. An approach to quantify biodegradation by
soil bacteria was developed, combining experiment with mathematical modelling. We performed in vitro assays to investigate
the potential and kinetics of the wild-type degrader, Burkholderia sp. strain LB400 (expressing bph) and the genetically modified Pseudomonas fluorescens strains F113pcb and F113L::1180 (expressing bph under different promoters) to metabolise individual congeners of polychlorinated biphenyls (PCBs). Kinetics of metabolism
was analysed using the Monod model. Results revealed similar patterns of degradable PCB congeners for LB400 and F113L::1180. The degree of PCB degradation
was comparable for LB400 and F113L::1180 but was much lower for F113rifpcb. In additional mesocosm experiments with PCB-contaminated
soil, the F113 derivatives demonstrated a good survival ability in willow (Salix sp.) rhizosphere. Strain F113L::1180 in combination with willow plants is expected to degrade a large spectrum of PCB congeners
in soil. The data from the experiments were used to calculate the time scale of the degradation process in a PCB-contaminated
soil. The uncertainty of the model predictions due to the uncertainties of experimental removal velocities and bacterial cell
density in soil was quantified. |
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Keywords: | Bacteria Cometabolism Genetically modified Biodegradation Modelling Monod kinetics PCB Rhizoremediation Soil contamination Willow |
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