Oxygen effect in heat-induced DNA damage |
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| Authors: | A V Chernikov S V Gudkov I N Shtarkman V I Bruskov |
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| Institution: | (1) Institute of Theoretica and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia;(2) Pushchino State University, Pushchino, Moscow Region, 142290, Russia |
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| Abstract: | The major kinds of heat-induced damage to DNA (depurination, guanine oxidation to 8-oxoguanine, cytosine deamination to uracil) were shown to depend in their extent on the oxygen content in solution. Formation of hydrogen peroxide in water upon heating was enhanced in the presence of D2O and decreased by various scavengers of singlet oxygen, corroborating the involvement of 1O2 in the thermal generation of reactive oxygen species. The aggregate data indicate that all kinds of heat-induced DNA damage in solution arise through this common mechanism. |
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| Keywords: | heat energy reactive oxygen species 8-oxoguanine depurination cytosine deamination |
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